mirna microarray analysis  (Qiagen)

Journal: Cell Communication and Signaling : CCS

Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization

doi: 10.1186/s12964-024-01570-5

Figure Lengend Snippet: MiR-6794-5p is upregulated within Bcl-w-derived exosomes. A Heatmap showing expressed exosomal miRNAs between vector and Bcl-w. B A549 cells were transfected with the negative control (NC), miR-1343-5p, miR-2861, miR-6794-5p, and miR-122-5p mimics, respectively. The mRNA expression levels of Twist, Zeb1, N-cad, and Oct4 by each miRNA were measured by qRT-PCR. The values were normalized to GAPDH. C Expression level of miR-6794-5p in exosomes isolated from conditioned media from Bcl-w-overexpressing A549 cells. D miR-6794-5p expression levels in Bcl-w overexpressing U251 and A549 cells. E The expression of miR-6794-5p in plasma of normal and patients with lung cancer (normal, n = 29; lung cancer, n = 24) was analyzed by qRT-PCR. The values were normalized to U6. Data are presented as mean ± SD after triplicate. * P < 0.05; ** P <0.01; *** P <0.001. Student's t-test

Article Snippet: MiRNA microarray analysis was performed using exosomes isolated from the conditioned media of U251 cells overexpressing control vector and Bcl-w. MiRNA microarray analysis was provided by MACROGEN (Korea).

Techniques: Derivative Assay, Plasmid Preparation, Transfection, Negative Control, Expressing, Quantitative RT-PCR, Isolation, Clinical Proteomics

Journal: Cell Communication and Signaling : CCS

Article Title: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization

doi: 10.1186/s12964-024-01570-5

Figure Lengend Snippet: miR-6794-5p directly inhibits the expression of SOCS1. A Venn diagram indicated target candidate genes of miR-6794-5p using TargetScan and miRWalk, which are miRNA target prediction sites. B, C After overexpression of the miR-6794-5p mimic in U251 ( B ) and A549 ( C ) cells, the mRNA expression of each of the candidate genes was confirmed by qRT-PCR. The values were normalized to GAPDH. D After miR-6794-5p was overexpressed in both cells, the level of SOCS1 protein was confirmed by Western blot analysis. β-actin was used for normalization. The experiment was repeated with triplicates and representative Western blotting images are shown. E, F Dual luciferase activity was examined after A549 cells were co-transfected with wild-type (WT) or mutant (Mut) vectors of the SOCS1 3’UTR in the presence or absence of the miR-6794-5p mimic, respectively. G Ago2-RNA immunoprecipitation (Ago2-IP) assay was performed in negative control (NC) or miR-6794-5p overexpressed A549 cells, and SOCS1 enrichment was confirmed by qRT-PCR. The values were normalized to GAPDH. H The mRNA expression of SOCS1 in plasma of normal and patients with lung cancer (normal, n = 23; lung cancer, n = 23) was analyzed by qRT-PCR. I Kaplan-Meier plots were used to compare survival rates between normal groups and lung adenocarcinoma patients. All data are presented as the mean ± S.D. after triplicate. * P < 0.05; ** P < 0.01; *** P < 0.001. Student’s t-test

Article Snippet: MiRNA microarray analysis was performed using exosomes isolated from the conditioned media of U251 cells overexpressing control vector and Bcl-w. MiRNA microarray analysis was provided by MACROGEN (Korea).

Techniques: Expressing, Over Expression, Quantitative RT-PCR, Western Blot, Luciferase, Activity Assay, Transfection, Mutagenesis, RNA Immunoprecipitation, Negative Control, Clinical Proteomics

Journal: Medicine

Article Title: Network pharmacology and bioinformatics study on the treatment of renal fibrosis with persicae semen-carthami flos drug pair

doi: 10.1097/MD.0000000000032946

Figure Lengend Snippet: The dataset GSE162794 has a total of 92 miRNAs upregulated and 79 miRNAs downregulated. The dataset GSE42716 has a total of 12 miRNAs upregulated and 1201 miRNAs downregulated.

Article Snippet: The dataset GSE42716 ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE42716 ) is a miRNA microarray analysis of UUO model mice studied by Keio University School of Medicine, which has a total of 8 samples, including 4 samples in the UUO group and 4 samples in the control group.

Techniques: